Light Chains from Slow-Twitch Muscle Myosin.

Dissociation of light chains from cardiac myosin.

The substrate, ATP, protects the active site of cardiac myosin during a 10-min treatment at 37 "C and neutral pH in the absence of divalent cations; under these conditions there is an approximate 20 % dissociation of light chain C1 and 60 % loss of light chain CZ with no corresponding decrease in myosin ATPase activity. Higher temperatures, the absence of divalent cations, increased treatment t...

Difference between Myosin Light and Heavy Chain Isoforms Patterns in Fast- and Slow-twitch Skeletal Muscle: Effect of Endurance Training

Synthesis of adult myosin light chains by embryonic muscle cultures.

Myosin light chain synthesis has been analyzed in cultures of fast and slow muscles from chicken and quail embryos. Synthesis was assayed by [35S]methionine incorporation and two-dimensional electrophoresis of total cell extracts. Our results show that differentiated cultures of embryonic anterior latissimus dorsi and pectoral muscles synthesize proteins that comigrate on two-dimensional gels w...

Synthesis of myosin heavy and light chains in muscle cultures

The weight ratio of myosin/actin, the myosin heavy chain content as the percentage of total protein (wt/wt), and the kinds of myosin light chains were determined in (a) standard muscle cultures, (b) pure myotube cultures, and (c) fibroblast cultures. Cells for these cultures were obtained from the breast of 11-day chick embryos. Standard cultures contain, in addition to myotubes, large numbers ...

Human ventricular/slow twitch myosin alkali light chain gene characterization, sequence, and chromosomal location.

The gene coding for the human ventricular/slow twitch myosin alkali light chain isoform was isolated and sequenced. It was found to contain a total of seven exons, the last of which is completely 3'-untranslated sequence. Comparison of this gene sequence with that of the various fast twitch skeletal isoform gene sequences revealed that the exon-intron arrangement is conserved within the myosin ...